技术文章您现在的位置:首页 > 技术文章 > 实验室常见荧光蛋白的激发波长和发射波长

实验室常见荧光蛋白的激发波长和发射波长

发布时间:2023-04-06   点击次数:227次

紫外荧光蛋白UV Proteins

ProteinExcitation Wavelength  Emission Wavelength       
Sirius355424
Sandercyanin375630
shBFP-N158S/L173I375458




蓝色荧光蛋白Blue Proteins

ProteinExcitation WavelengthEmission Wavelength
Azurite383450
EBFP2383448
mKalama1385456
mTagBFP2                       399454
TagBFP402457
shBFP401458

青色荧光蛋白Cyan Proteins

ProteinExcitation WavelengthEmission Wavelength
ECFP433475
Cerulean433475
mCerulean3433475
SCFP3A433474
CyPet435477
mTurquoise434474
mTurquoise2434474
TagCFP458480
mTFP1462492
monomeric Midoriishi-Cyan470496
Aquamarine430474

绿色荧光蛋白Green Proteins

ProteinExcitation WavelengthEmission Wavelength
TurboGFP482502
TagGFP2483506
mUKG483499
Superfolder GFP485510
Emerald487509
EGFP488507
Monomeric Azami Green   492505
mWasabi493509
Clover505515
mNeonGreen506517
NowGFP494502
mClover3506518

黄色荧光蛋白Yellow Proteins

ProteinExcitation WavelengthEmission Wavelength
TagYFP508524
EYFP513527
Topaz514527
Venus515528
SYFP2515527
Citrine516529
Ypet517530
lanRFP-ΔS83                      521592
mPapaya1530541
mCyRFP1528594

桔色荧光蛋白Orange Proteins

ProteinExcitation WavelengthEmission Wavelength
Monomeric Kusabira-Orange548559
mOrange548562
mOrange2549565
mKOκ551563
mKO2551565

红色荧光蛋白Red Proteins

ProteinExcitation WavelengthEmission Wavelength
TagRFP555584
TagRFP-T555584
RRvT556583
mRuby558605
mRuby2559600
mTangerine                            568585
mApple568592
mStrawberry574596
FusionRed580608
mCherry587610
mNectarine558578
mRuby3558592
mScarlet569594
mScarlet-I569593

远红荧光蛋白Far Red Proteins

ProteinExcitation WavelengthEmission Wavelength
mKate2588633
HcRed-Tandem590637
mPlum590649
mRaspberry598625
mNeptune600650
NirFP605670
TagRFP657                            611657
TagRFP675598675
mCardinal604659
mStable597633
mMaroon1609657
mGarnet2598671

近红荧光蛋白Near IR Proteins

ProteinExcitation WavelengthEmission Wavelength
iFP1.4684708
iRFP713 (iRFP)                     690713
iRFP670643670
iRFP682663682
iRFP702673702
iRFP720702720
iFP2.0690711
mIFP683704
TDsmURFP642670
miRFP670642670

Sapphire-type Proteins

ProteinExcitation WavelengthEmission Wavelength
Sapphire399511
T-Sapphire                             399511
mAmetrine406

526

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


上海路阳仪器有限公司

上海路阳仪器有限公司

地址:上海市松江区新桥镇泗砖南路255弄120号

主营产品:黑光灯,探伤灯,紫外线探伤灯,LED磁粉探伤灯,荧光检漏灯,荧光检漏剂,检漏手电筒,脱脂检测灯

© 2023 版权所有:上海路阳仪器有限公司  备案号:沪ICP备10002130号-7  总访问量:189689  站点地图  技术支持:化工仪器网  管理登陆

QQ在线客服
联系方式

18917039682

19921813005

86-021-64199903