摘要：
2026 年，海南大学热带农林学院（国家热带作物育种重点实验室）联合海南省天然橡胶协同创新中心在《BioDesign Research》期刊发表文献《Laticifer-specific engineering of rubber biosynthesis in Taraxacum kok-saghyz》（南京农业大学主办、美国科学促进会（AAAS）合作出版，已被 ESCI、PubMed Central 收录），文献中明确指出利用 LUYOR-3260 荧光蛋白激发光源可高效筛选 GFP 标记的转基因橡胶树愈伤组织，为橡胶树乳管（laticifer）特异性遗传改良及天然橡胶生物合成研究提供关键技术支撑。
海南大学团队利用 LUYOR-3260 荧光蛋白激发光源筛选 GFP 标记的转基因橡胶树愈伤组织
2026 年，海南大学主导的研究团队在《BioDesign Research》发表研究成果，系统揭示了橡胶草（Taraxacum kok-saghyz，TKS）乳管特异性启动子 pTkTHFI 的鉴定及其在橡胶树（Hevea brasiliensis）中的跨物种表达活性。该研究中，LUYOR-3260 荧光蛋白激发光源作为关键工具，承担了 GFP 标记转基因橡胶树愈伤组织的筛选任务，其稳定的荧光识别能力和高效的筛选效率为获得阳性转基因材料、推进体细胞胚胎发生与植株再生奠定了基础。
海南大学热带农林学院依托国家热带作物育种重点实验室，围绕天然橡胶等热带战略作物的重大需求，在产胶植物乳管发育、天然橡胶生物合成与遗传改良等前沿领域开展系统研究。该研究基于橡胶草全生育期转录组图谱，鉴定出 1267 个乳管偏好表达基因，并从中筛选出在乳管中严格特异性表达的 pTkTHFI 启动子，为橡胶树的精准遗传改良提供了重要理论支撑和技术储备。
在该实验中，研究团队将 pTkTHFI 启动子与 GUS 报告基因融合，构建含独立 GFP 转录单元的表达载体并转化橡胶树优良品种 RY7-33-97；转化后的抗性愈伤组织通过 LUYOR-3260 荧光蛋白激发光源可视化 GFP 绿色荧光进行筛选，阳性愈伤组织经体细胞胚胎发生途径诱导胚胎，再于生根培养基上诱导生根；转基因根系经 GUS 组织化学染色与碘-溴（I-Br）染色分析，证实 pTkTHFI 启动子在橡胶树根系乳管中保持特异性表达活性。
LUYOR-3260 系列荧光蛋白激发光源是一款专为转基因生物筛选设计的便携式检测工具，采用手电筒式设计，配备多颗大功率 LED 光源，电池供电，照射面积大、光斑均匀，能够稳定激发绿色荧光蛋白（GFP）与红色荧光蛋白（DsRed）的荧光；无需底物显色即可直接观测，即开即用、系统稳定，既适用于实验室常规筛选，也便于田间野外原位检测；并提供多种波长型号可选（365 nm 紫外、450 nm 深蓝、488 nm 浅蓝、505 nm 青色、520 nm 绿色），适配不同荧光蛋白的激发需求。该产品操作简便、荧光信号识别精准，已被广泛应用于研究所、高校等生物研究部门，成为转基因植物、动物和微生物筛选的重要工具。
原文段落
Rubber dandelion (Taraxacum kok-saghyz, TKS) is a promising alternative source of natural rubber, which is synthesized in specialized laticifer cells. While genetic engineering of TKS holds promise for enhancing rubber yield, constitutive transgene expression often leads to undesirable pleiotropic effects. Therefore, identification of laticifer-specific promoters, especially those active throughout all stages of rubber accumulation, is essential for precise genetic manipulation of rubber biosynthesis. Here, we performed a comprehensive transcriptomic analysis across nine developmental stages and five tissue types in an elite TKS accession. This identified 1267 laticifer-preferentially expressed genes, 261 of which were constitutively overrepresented in laticifers at every developmental stage. Promoters from four candidate laticifer-specific genes were cloned and evaluated in stably transformed TKS. One promoter, pTkTHFI, drove expression exclusively in laticifers. We further showed that pTkTHFI retains its laticifer-specific activity in transgenic rubber tree (Hevea brasiliensis), highlighting its utility for targeted genetic improvement in both rubber-producing species. Finally, by overexpressing HbSRPP1, a key component of rubber transferase complex, using pTkTHFI versus the constitutive p35S promoter in TKS, we demonstrate that tissue-specific engineering enhances plant growth and rubber yield more effectively than constitutive expression, underscoring the advantage of precise genetic control for TKS improvement.
Rubber tree transformation was performed as previously described [32]. The resistant calli were screened using the LUYOR-3260 fluorescent protein excitation light source (LUYOR, Shanghai, China). The GFP-positive lines were subjected to embryogenesis. Finally, the regenerated embryos placed on the rooting medium for root induction. Transgenic roots were dissected for histology analysis.
文献：10.1016/j.bidere.2026.100091
