UCL-3500紫外交联仪在紫外线诱导突变中的应用
摘要：
2025 年，北京化工大学化学工程学院联合生命科学与技术学院绿色生物制造国家重点实验室在《Biochemical Engineering Journal》期刊发表文献《Screening of high-yielding β-farnesene Saccharomyces cerevisiae strains and quantification of fermentation processes: A new fluorescence-based high-throughput screening and quantification platform》，该文献明确指出利用紫外交联仪 UCL-3500 可高效开展紫外线诱导突变实验，为高产 β- 法尼烯酿酒酵母菌株的筛选及发酵过程量化提供了关键技术支撑，显著提升了紫外线诱导突变筛选的效率与准确性。
紫外交联仪 UCL-3500 助力紫外线诱导突变筛选高产酵母菌株
2025 年，北京化工大学主导的研究团队在《Biochemical Engineering Journal》发表重要成果，成功建立了基于荧光探针的高通量筛选与定量平台，实现了从紫外线诱导突变菌株筛选到规模化发酵过程监测的全流程优化。在该研究中，紫外交联仪 UCL-3500 作为核心实验设备，承担了酿酒酵母的紫外线诱导突变任务，其稳定的紫外线输出、精准的照射时间控制能力，为构建高质量突变体库、高效筛选高产 β- 法尼烯菌株奠定了坚实基础。
北京化工大学化学工程学院与绿色生物制造国家重点实验室是我国生物化工领域的重要研究阵地，围绕绿色可持续生产的重大战略需求，在微生物代谢工程、生物催化与转化、发酵过程优化等前沿领域开展系统研究。重点方向包括萜类化合物生物合成、微生物菌株改良、高效检测技术开发等，曾在多个国际优秀期刊发表多项突破性成果，为生物制造产业的升级发展提供了重要理论支撑和技术储备。
在该实验中，研究团队以产 β- 法尼烯工程酿酒酵母菌株 BUHY0003 为研究对象，采用紫外交联仪 UCL-3500 开展紫外线诱导突变实验。研究人员将 5mL 菌株悬液置于无菌培养皿中，加入无菌磁力搅拌子使菌液形成均匀薄层，随后将培养皿置于紫外交联仪 UCL-3500 紫外线灯下方约 20cm 处，设置 0、20、25、30 等多个照射时间梯度进行紫外线诱导突变处理，每个处理组均设置三次重复。紫外线诱导突变处理后，将菌液在黑暗条件下离心收集，重悬于 YPD 培养基中恢复培养 2 小时，再涂布于 YPD 琼脂培养基上培养，通过计算菌落存活率确定紫外线诱导突变参数。最终发现，当紫外交联仪 UCL-3500 的照射时间为 50 秒时，菌株致死率达到 94.76%，此条件下既能保证足够的突变负荷，又能维持突变体库的实用规模，是开展紫外线诱导突变的设置。
通过紫外交联仪 UCL-3500 完成紫外线诱导突变后，研究团队利用构建的荧光高通量筛选平台对突变菌株进行筛选，成功获得高产突变株 BUHY0004，其 β- 法尼烯产量较原始菌株提升 37.34%。后续经 5L 和 30L 补料分批发酵验证，该突变株表现出良好的规模化生产性能，产量分别达到 35.1g/L 和 33.3g/L，充分证明了紫外交联仪 UCL-3500 在紫外线诱导突变菌株改良中的可靠性与高效性。
紫外交联仪 UCL-3500 是一款专为微生物紫外线诱导突变设计的专业设备，采用稳定的紫外线光源设计，可提供均匀、高强度的紫外线照射，确保紫外线诱导突变的有效性。设备支持灵活的照射时间调节与能量调控，能满足不同菌株的紫外线诱导突变需求，可精准控制突变强度，为筛选有益突变体创造有利条件。操作简便，无需复杂的前期调试，适用于实验室大规模突变体库构建。其适配性强，可广泛应用于酿酒酵母、大肠杆菌等多种微生物的紫外线诱导突变实验，为代谢工程、菌株改良、发酵工业等领域的研究提供强有力的技术支持。目前，该设备已成为高校、科研院所开展微生物紫外线诱导突变相关研究的重要工具，相关研究成果已在多个国际优秀期刊发表。
原文相关段落摘录：
Saccharomyces cerevisiae is regarded as one of the most important model strain and industrial workhorses in metabolic engineering and biotechnology, and has been extensively employed for the manufacturing of natural products. Although substantial advances in genome editing and rational design have been achieved, growth retardation in scale-up fermentations is frequently observed in engineered yeasts owing to the complex regulation of central carbon metabolism. In this context, ultraviolet (UV) mutagenesis is recognized as a powerful complementary strategy, enabling the rapid generation of extensive mutant libraries and facilitating the identification of beneficial alleles.
UV mutagenesis and 96-well plate screeningIn this study, the engineered S. cerevisiae strain BUHY0003 used for producing β-farnesene was constructed according to our previous work. A mutagenesis chamber (UCL-3500, Shanghai Luyang Biotechnology Co., Ltd., China) equipped with two 18 W UV lamps was used as the light source and was preheated for 20 min before use. 5 mL of BUHY0003 strain suspension were placed in a sterile 9 cm culture dish. A sterile 1.5 cm magnetic stir bar was added, and the dish was gently agitated on a shaker to form a uniform thin layer. The dish was positioned approximately 20 cm below the UV lamps and was irradiated at normal incidence in triplicate. Irradiation times were set to 0, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, and 80 s. Following irradiation, cells were collected by centrifugation at 4000 × g for 4 min in the dark. The supernatant was discarded, and the cells were resuspended in YPD medium followed by a 2-hour recovery incubation at 30◦C with shaking at 200 rpm. 100 μL of the suspension were spread onto YPD agar medium and were incubated inverted at 30◦C for 96 h. Under sterile conditions, colonies were counted and lethality was calculated.
