激发光源LUYOR-3415 RG筛选GFP转基因大豆毛状根
2025 年，华中农业大学作物遗传改良国家重点实验室、植物科学技术学院联合湖北省洪山实验室、吉林省农业科学院农业生物技术吉林省重点实验室在《Nature Plants》期刊发表文献《The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1》（IF=20.1，中科院一区 Top 期刊），文献中明确指出利用 LUYOR-3415 荧光蛋白激发光源可高效筛选 GFP 标记的转基因大豆毛状根，为解析大豆根瘤共生固氮的分子机制提供关键技术支撑。
华中农业大学团队利用 LUYOR-3415RG 荧光蛋白激发光源筛选 GFP 标记的转基因大豆毛状根
2025 年，华中农业大学主导的研究团队在《Nature Plants》发表重磅成果，揭示了铁传感器 BTSa 通过单泛素化修饰 NSP1a 调控大豆根瘤发育的核心机制。该研究中，LUYOR-3415RG 荧光蛋白激发光源作为关键工具，承担了 GFP 标记转基因大豆毛状根的筛选任务，其稳定的荧光识别能力和高效的筛选效率为实验成功奠定了基础。
华中农业大学作物遗传改良国家重点实验室是我国作物科学研究的核心阵地，围绕农业可持续发展的重大战略需求，在作物遗传育种、植物与微生物互作、营养信号调控等前沿领域开展系统研究。重点方向包括大豆共生固氮机制、作物营养高效利用、转基因技术创新等，曾在 Nature 系列、Cell 等国际期刊发表多项突破性成果，为保障国家粮食安全提供重要理论支撑和技术储备。
在该实验中，研究团队采用农杆菌介导的大豆毛状根转化技术，将 UBIpro:BTSa–4×MYC、UBIpro:NSP1a–4×MYC、NSP1apro:NSP1aWT–4×MYC 等载体（含 GFP 报告基因 cassette）转入大豆毛状根；转化后的复合植株在培养箱中培养 7 天后，转移至含不同铁浓度的 BD–LN 营养液湿润的蛭石中预处理 3 天，再接种慢生根瘤菌 USDA110；通过 LUYOR-3415 荧光蛋白激发光源可视化 GFP 绿色荧光，快速筛选阳性转基因毛状根，随后进行根瘤计数、氮酶活性检测及分子机制分析。
LUYOR-3415RG 荧光蛋白激发光源是一款专为转基因生物筛选设计的快速检测工具，采用充电电池供电，双波长激发设计适配绿色 / 红色荧光蛋白，续航能力强，既适用于实验室常规筛选，也满足野外原位观察需求；配合专用支架可实现长时间稳定观察，避免人工手持疲劳。其单个波长配备 6×3W LED 发光模块，照射面积广，能快速完成大批量样品筛选，显著提升实验效率；可灵活选配不同波长模块，兼容 GFP、eGFP、DsRed、mCherry、TdTomato 等多种荧光蛋白，满足多元化实验需求。该产品操作简便、荧光信号识别精准，已成为高校、科研院所转基因实验的核心工具，累计有近千篇高水平科研文献发表。
原文段落
Legumes form root nodules with symbiotic nitrogen-fixing rhizobacteria, which require ample iron to ensure symbiosis establishment and efficient nitrogen fixation. The functions and mechanisms of iron in nitrogen-fixing nodules are well established. However, the role of iron and the mechanisms by which legumes sense iron and incorporate this cue into nodulation signalling pathways remain unclear. Here we show that iron is a key driver of nodulation because symbiotic nodules cannot form without iron, even under conditions of sufficient light and low nitrogen. We further identify an iron optimum for soybean nodulation and the iron sensor BRUTUS A (BTSa) which acts as a hub for integrating iron and nodulation cues. BTSa is induced by rhizobia, binds to and is stabilized by iron. In turn, BTSa stabilizes and enhances the transcriptional activation activity of pro-nodulation transcription factor NSP1a by monoubiquitination from its RING domain and consequently activates nodulation signalling. Monoubiquitination of NSP1 by BTS is conserved in legumes to trigger nodulation under iron sufficiency. Thus, iron status is an essential cue to trigger nodulation and BTSa integrates cues from rhizobial infection and iron status to orchestrate host responses towards establishing symbiotic nitrogen fixation.
Soybean hairy-root transformation and B. diazoefficiens inoculation assayHairy-root transformation with Agrobacterium rhizogenes K599 was carried out as previously described. In brief, 3-day-old seedlings were used for transformation. Transgenic composite plants were transferred to vermiculite in a growth chamber (16 h light/8 h dark, 25 °C, 50% relative humidity). For iron treatments, 7-day-old composite plants were transferred to vermiculite moistened with BD–LN nutrient solution with 10 µM Fe citrate or 200 µM AA-DP/2,2′-bipyridine for 3 d, and then inoculated with B. diazoefficiens USDA110, and hairy-root samples were collected for further analysis. Transgenic hairy roots transformed with UBIpro:BTSa–4×MYC, UBIpro:NSP1a–4×MYC, NSP1apro:NSP1aWT–4×MYC or NSP1apro:NSP1a5KR–4×MYC (with GFP in another cassette) were screened using a portable fluorescence lamp (LUYOR-3415, LUYOR) to visualize GFP fluorescence.
原文献：10.1038/s41477-024-01896-5
