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sfGFP荧光蛋白的激发波长和发射波长

发布时间:2022-10-11   点击次数:263次

                                                                                                                            sfGFP荧光蛋白的激发波长和发射波长


sfGFP的激发波长主峰在488nm,发射波长主峰在507nm。

上海路阳仪器有限公司现货销售进口便携式荧光蛋白激发光源,又叫荧光蛋白观察灯、GFP激发光源,这个便携式光源能够激发GFPEGFPBFPCFPYFPRFPDsredmcherry等各种荧光蛋白的荧光,常备现货的有绿色荧光蛋白、红色荧光蛋白、黄色荧光蛋白激发光源咨

常用的报告基因包括:lacZEgfp zsGreenEYFPDsRed (DsRed2)mRFPmCherryHuCD2Thy1.1HA tagFLAG tagLuciferase等。zsGreenEGFP, EYFP都是绿色荧光。EGFPEYFP很难区分开。zsGreen是一个比较稳定的绿色荧光蛋白。DsRedTdTomato都是红色荧光。TdTomato是一个信号非常强的荧光蛋白,对细胞/小鼠也没有明显的毒性。mRFPmCherry是两个非常好的荧光蛋白,需要530nm的激发光,所以可以用LUYOR-3415RG荧光蛋白观察灯激发,能够用肉眼观察到荧光蛋白在小鼠上的表达。

上海路阳仪器有限公司销售美国路阳便携式荧光蛋白激发光源、荧光手电筒,能够激发各种荧光蛋白在动植物上的转基因表达,如果您要观察GFP荧光蛋白在植物叶片、愈伤、种子、根系的转基因表达。上海路阳提供样机免费试用,满意付款。我们还在北京、郑州、海口、武汉、哈尔滨设有代理商,可以提供上门演示。详情请惦记店铺查询

sfGFP--Superfolder GFP

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.

What is Superfolder GFP?

Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer. +.


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