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紫外交联仪在实验中的具体应用有哪些?

发布时间:2022-09-01   点击次数:96次

紫外交联仪的应用/ UV crosslinker APPLICATIONS

  紫外交联仪UCL-3200/3500 也可以在需要计量紫外线强度时使用。短波 (254nm)、中波 (312nm) 或长波 (365nm) 紫外管均可用于此类应用。在 INTENSITY 模式下,该装置还可用于监测室内的强度。然而,在这种模式下,灯一直亮着,直到按下“小数点"按钮。

  The UCL-3200/3500 can also be used whenever a metered UV dosage is required. Either short wave (254nm), medium wave (312nm), or long wave (365nm) UV tubes may be used for such applications. In the INTENSITY mode, the unit may also be used to monitor intensity inside the chamber. In this mode, however, the lamps remain on until either the "."’ button is pressed or the power is turned off。

  常用于切割 DNA/ NICKING DNA;基因图谱/GENE MAPPING;测试和筛选 RecA TESTING AND SCREENING RecA;紫外线杀菌/UV STERILIZATION;消除 PCR* 污染物/ELIMINATING PCR* CONTAMINANTS

  紫外交联仪UCL-3200/3500 可用于在 Northern、Southern、狭缝或斑点印迹以及菌落或斑块剥离后将核酸共价结合到膜上。研究表明,120 mJ/cm2 是在任何这些程序中将 DNA 或 RNA 附着到尼龙或硝酸纤维素膜上的佳剂量。为了在 DNA 或 RNA 转移步骤后获得佳结果,应在膜干燥后将膜放入 UCL-3200/3500 室内。放置膜,使核酸附着的一面朝上(即朝向光源),以便直接照射核酸。选择一个能量值,然后按“START"以启动所需功能。

  The UCL-3200/3500 can be used to covalently bind nucleic acids to membranes after Northern, Southern, slot or dot blotting, and colony or plaque lifts. Studies indicate that 120 mJ/cm2 is an optimal dosage for attachment of DNA or RNA in any of these procedures to nylon or nitrocellulose membranes. In order to obtain the best results following the DNA or RNA transfer step, the membrane should be placed inside the UCL-3200/3500 chamber after the membrane is dried. Place the membrane with the side to which the nucleic acids are attached facing upwards (i.e. towards the light source) so that the nucleic acids are directly irradiated. Select an energy value  followed by ‘‘START’’ to initiate the desired function.

紫外交联仪UCL-3200/3500操作面板

紫外交联仪UCL-3200/3500操作面板

切割 DNA/ NICKING DNA

紫外交联仪UCL-3200/3500 可用于在琼脂糖凝胶中切割溴化乙锭染色的 DNA,以代替脱嘌呤洗涤液。

The UCL-3200/3500 can be used to nick the ethidium-bromide-stained DNA in agarose gels in place of a depurination wash.

基因图谱/GENE MAPPING

紫外交联仪UCL-3200/3500 可用于在用限制酶处理之前产生胸腺嘧啶二聚体。在识别位点处或附近形成二聚体会抑制切割。

The UCL-3200/3500 can be used to create thymine dimers prior to treatment with a restriction enzyme. The formation of dimers at or near the recognition site inhibits cleavage 。

测试和筛选 RecA TESTING AND SCREENING RecA

紫外交联仪UCL-3200/3500 可用于检测和筛选 RecA 突变,该突变可以防止紫外线引起的细胞损伤修复,从而延缓其生长。

The UCL-3200/3500 can be used for testing and screening RecA mutation which can prevent repair of UV-induced damage on cells, thereby retarding their growth.

紫外线杀菌/UV STERILIZATION

当紫外交联仪 UCL-3200/3500 配备短波 (254nm) 管时,可以为实验室目的进行各种灭菌过程。

When the UCL-3200/3500 is equipped with short wave (254nm) tubes, various sterilization processes can be undertaken for laboratory purposes .

消除 PCR* 污染物/ELIMINATING PCR* CONTAMINANTS

紫外交联仪UCL-3200/3500 可用于消除 PCR 测试期间可能出现的污染物。

The UCL-3200/3500 can be used to eliminate contaminants that may occur during PCR tests.

紫外交联仪UCL-3200/3500


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